Composite

Part:BBa_K5159020:Design

Designed by: Tianyue WU   Group: iGEM24_HKUST   (2024-09-30)


LEU2 marker expression cassette


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 35
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 35
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 35
    Illegal BglII site found at 2267
    Illegal XhoI site found at 981
    Illegal XhoI site found at 1010
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 35
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 35
    Illegal NgoMIV site found at 123
    Illegal AgeI site found at 1595
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 641
    Illegal BsaI site found at 924
    Illegal BsaI.rc site found at 839
    Illegal SapI site found at 1392


Design Notes

None


Source

Most of the sequence is identified from the genome of Yarrowia lipolytica. The cassette was synthesized by commercial provider Integrated DNA Technology (IDT) in one fragment.

References